Journal: Biochimica et biophysica acta

Article Title: Endothelial Nlrp3 Inflammasome Activation Associated with Lysosomal Destabilization during Coronary Arteritis

doi: 10.1016/j.bbamcr.2014.11.012

Figure Lengend Snippet: MVECs were stimulated with or without LCWE (10 μg/ml, 8 h) in the presence of PBS (Vehl: vehicle), potassium channel blocker glibenclamide (GLY, 10 μM, Sigma) or ROS scavenger N-acetyl-L-cysteine (NAC, 10 μM, Sigma). (A) Representative Western blot documents and summarized data showing the effects of glibenclamide or N-acetyl-L-cysteine on the expression of pro-caspase-1, cleaved caspase-1 and β-actin (n = 3). (B) IL-1β production measurement in MVECs by ELISA (n=6). * P < 0.05, LCWE vs. control; # P < 0.05 vs. vehicle+LCWE.

Article Snippet: Thus, these results exclude the involvement of potassium ions external flow or ROS in LCWE-induced activation of Nlrp3 inflammasomes. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 5 caption a7 caption a8 Effects of potassium channel blockade or ROS scavenging on LCWE-induced activation of Nlrp3 inflammasomes in MVECs MVECs were stimulated with or without LCWE (10 μg/ml, 8 h) in the presence of PBS (Vehl: vehicle), potassium channel blocker glibenclamide (GLY, 10 μM, Sigma) or ROS scavenger N-acetyl-L-cysteine (NAC, 10 μM, Sigma). (A) Representative Western blot documents and summarized data showing the effects of glibenclamide or N-acetyl-L-cysteine on the expression of pro-caspase-1, cleaved caspase-1 and β-actin (n = 3). (B) IL-1β production measurement in MVECs by ELISA (n=6).

Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Separate and combined effects of K Ca and K ATP channel blockade with NOS inhibition on cutaneous vasodilation and sweating in older men during heat stress

doi: 10.1152/ajpregu.00075.2019

Figure Lengend Snippet: Time course changes (n = 11 participants) in cutaneous vascular conductance (CVC%max) during nonheated rest (25°C) and rest (R35), exercise, and recovery in the heat (35°C) (A) and mean (solid bars) and individual (○) responses for each skin site during nonheated rest (B) and at rest (C), end of exercise (D), and end of recovery (E) in the heat. Six skin sites were continuously treated with either 1) lactated Ringer solution [control (CON); ●], 2) 10 mM NG-nitro-l-arginine methyl ester (l-NAME; nitric oxide synthase inhibitor; pink circles), 3) 50 mM tetraethylammonium (TEA; Ca2+-activated K+ channel blocker; green circles), 4) 5 mM glybenclamide (GLY; ATP-sensitive K+ channel blocker; red circles), 5) 50 mM TEA + 10 mM l-NAME (TEA + l-NAME; orange circles), and, 6) 5 mM GLY + 10 mM l-NAME (GLY + l-NAME; blue circles). Main interaction effect for site × time (P = 0.007). Data are presented as means ± 95% confidence interval. *Significantly different at P ≤ 0.05. BL, baseline.

Article Snippet: The sites were perfused with either 1 ) lactated Ringer solution [control (CON)], 2 ) 10 mM N G -nitro- l -arginine methyl ester [ l -NAME; NOS inhibitor (Sigma-Aldrich, St. Louis, MO)], 3 ) 50 mM tetraethylammonium [TEA; K Ca channel blocker (Sigma-Aldrich)], 4 ) 5 mM glybenclamide [GLY; K ATP channel blocker (Sigma-Aldrich)], 5 ) 50 mM TEA + 10 mM l -NAME [TEA + l -NAME], or 6 ) 5 mM GLY + 10 mM l -NAME [GLY + l -NAME].

Techniques:

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Separate and combined effects of K Ca and K ATP channel blockade with NOS inhibition on cutaneous vasodilation and sweating in older men during heat stress

doi: 10.1152/ajpregu.00075.2019

Figure Lengend Snippet: Time course changes (n = 13 participants) in local sweat rate during nonheated rest (25°C) and rest (R35), exercise, and recovery in the heat (35°C) (A) and mean (solid bars) and individual (○) responses for each skin site during nonheated rest (B) and at rest (C), end of exercise (D), and end of recovery (E) in the heat. Six skin sites were continuously treated with either 1) lactated Ringer solution [control (CON); ●], 2) 10 mM NG-nitro-l-arginine methyl ester (l-NAME, nitric oxide synthase inhibitor; pink circles), 3) 50 mM tetraethylammonium (TEA; Ca2+-activated K+ channel blocker; green circles), 4) 5 mM glybenclamide (GLY; ATP-sensitive K+ channel blocker; red circles), 5) 50 mM TEA + 10 mM l-NAME (TEA + l-NAME; orange circles), and 6) 5 mM GLY + 10 mM l-NAME (GLY + l-NAME; blue circles). Main interaction effect for site × time (P = 0.031). Data are presented as mean ± 95% confidence interval. *Significantly different at P ≤ 0.05. BL, baseline.

Article Snippet: The sites were perfused with either 1 ) lactated Ringer solution [control (CON)], 2 ) 10 mM N G -nitro- l -arginine methyl ester [ l -NAME; NOS inhibitor (Sigma-Aldrich, St. Louis, MO)], 3 ) 50 mM tetraethylammonium [TEA; K Ca channel blocker (Sigma-Aldrich)], 4 ) 5 mM glybenclamide [GLY; K ATP channel blocker (Sigma-Aldrich)], 5 ) 50 mM TEA + 10 mM l -NAME [TEA + l -NAME], or 6 ) 5 mM GLY + 10 mM l -NAME [GLY + l -NAME].

Techniques:

Journal: The Journal of Immunology Author Choice

Article Title: Salmonella Flagellin Activates NAIP/NLRC4 and Canonical NLRP3 Inflammasomes in Human Macrophages

doi: 10.4049/jimmunol.2000382

Figure Lengend Snippet: Recombinant PrgI triggers the NAIP/NLRC4 inflammasome, whereas flagellin activates the NAIP/NLRC4 and the NLRP3 inflammasome in THP-1 cells. Parental wild-type THP-1 ( A , B , and D ), NLRC4 KO (A and B), NAIP KO ( C ), or NLRP3 KO (D) cells were transfected with PrgI or flagellin (A–D) or stimulated with LPS+ nigericin (B–D) in the presence of NLRP3 inhibitors MCC950 (B and C) or glybenclamide (B). ASC speck formation (B–D) and IL-1β (A) and IL-18 (A–D) release were determined at 4 h poststimulation. Mean with SD of at least three individual experiments (two replicates/experiment) is displayed. Statistical analysis was performed using two-way ANOVA followed by a Bonferroni posttest. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Native ultrapure S. Typhimurium flagellin (tlrl-epstfla-5), ultrapure Bacillus subtilis flagellin (tlrl-pbsfla), the NLRP3 inhibitors MCC950 (inh-mcc), and glybenclamide (tlrl-gly) were purchased from InvivoGen.

Techniques: Recombinant, Transfection